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  1. Restriction enzyme data file used by the programs MAP, MAPSORT and
  2. MAPPLOT.  A complete format description of this file may be found in the
  3. Data Files volume of the Data Reference Set.
  4.  
  5. Only one enzyme per recognition specificity is used when the
  6. '*' is pressed to select all default enzymes. In addition,
  7. enzymes that are commercially available but are not unique in
  8. their recognition specificity can be selected when explicitly named.
  9.  
  10. We are deeply grateful to Dr. Rich Roberts for his help with the
  11. maintenance of our enzyme data.
  12.  
  13. REBASE version 9201
  14.  
  15. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
  16.      + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 
  17.               Dr. Richard J. Roberts Restriction Enzyme Database
  18.              Copyright (c) Cold Spring Harbor Laboratory 1992
  19.                         All rights reserved.
  20.      + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 
  21. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
  22.  
  23. Rich Roberts                                          Jan 01 92
  24.  
  25. Commercial sources of restriction enzymes are abbreviated as follows:
  26.  
  27.         A    Amersham (12/91)
  28.         B    BRL (6/91)
  29.         D    Palliard Chemical (5/91)
  30.         E    Stratagene (9/91)
  31.         F    Fermentas (6/91)
  32.         H    American Allied (6/91)
  33.         I    IBI (6/91)
  34.         J    Janssen Biochimica (2/91)
  35.         K    Takara (10/91)
  36.         L    Northumbria Biologicals Ltd. (10/91)
  37.         M    Boehringer Mannheim (9/91)
  38.         N    New England Biolabs (7/91)
  39.         O    Toyobo (6/91)
  40.         P    PL-Pharmacia-LKB (9/91)
  41.         Q    Molecular Biology Resources (10/91)
  42.         R    Promega Corporation (10/91)
  43.         S    Sigma (6/91)
  44.         U    USB (10/91)
  45.         V    Serva (2/91)
  46.         W    ILS (5/91)
  47.         X    New York Biolabs (4/91)
  48.          Y    P.C. Bio (9/91)
  49.  
  50. Addresses for many of these sources can be obtained with the command:
  51.  
  52.                     fetch enz_sources.txt
  53.  
  54. Please note:
  55.  
  56.      REBASE commercial source abbreviation "G" has been deleted, because
  57. the company called BioExcellence (formerly Anglian), has gone out of business.
  58.  
  59. The numbers at the end of each line point to literature references for
  60. that enzyme. The file containing the literature references can be
  61. obtained with the command:
  62.  
  63.                     fetch enz_refs.txt
  64.  
  65.  ..
  66. ;AatI      3 AGG'CCT        0           !  Eco147I,StuI                      >OU    1
  67. AatII      5 G_ACGT'C      -4           !                                    >EJLMNOPRSUVX    1
  68. AccI       2 GT'mk_AC       2           !                                    >ABDEIJKLMNOPQRSUVXY    2
  69. ;AccII     2 CG'CG          0           !  Bsp50I,BstUI,MvnI,ThaI            >DEJKQVXY    2,3
  70. ;AccIII    1 T'CCGG_A       4           !  BseAI,BsiMI,Bsp13I,BspEI,Kpn2I,MroI  >DEJKQRVY    4,5
  71. ;Acc65I    1 G'GTAC_C       4           !  Asp718I,KpnI                      >DFNY    6
  72. ;AccB1I    1 G'GyrC_C       4           !  BanI,Eco64I                       >DY    7
  73. ;AccB7I    7 CCAn_nnn'nTGG  -3          !  PflMI,Van91I                      >DY    8
  74. AciI       1 C'CGC          4           !                                    >N    9
  75. ;AcyI      2 Gr'CG_yC       2           !  BbiII,BsaHI,Hin1I                 >EMRV    10
  76. ;AfaI      2 GT'AC          0           !  Csp6I,RsaI                        >K    11
  77. AflII      1 C'TTAA_G       4           !  BfrI,Esp4I                        >ABJKNU    12
  78. AflIII     1 A'CryG_T       4           !                                    >BJMNU    12
  79. AgeI       1 A'CCGG_T       4           !                                    >N    13
  80. ;AhaIII    3 TTT'AAA        0           !  DraI                              >    14
  81. AluI       2 AG'CT          0           !                                    >ABDEFHIJKLMNOPQRSUVXY    15,16,17,18
  82. AlwI       9 GGATCnnnn'n_   1           !                                    >NU    19
  83. ;Alw21I    5 G_wGCw'C      -4           !  AspHI,HgiAI                       >DFY    20
  84. ;Alw26I    6 GTCTCn'nnnn_   4           !  BsmAI                             >DFRY    21
  85. ;Alw44I    1 G'TGCA_C       4           !  ApaLI,SnoI,VneI                   >DFORUY    20
  86. AlwNI      6 CAG_nnn'CTG   -3           !                                    >NU    22
  87. ;Ama87I    1 C'yCGr_G       4           !  AvaI,BcoI,Eco88I,NspIII           >DY    7
  88. ;AocI      2 CC'TnA_GG      3           !  AxyI,Bse21I,Bsu36I,CvnI,Eco81I,MstII,SauI  >E    23
  89. ;AosI      3 TGC'GCA        0           !  AviII,FdiII,FspI,MstI             >J    24
  90. ApaI       5 G_GGCC'C      -4           !  Bsp120I                           >BDEIJKLMNOPRUVXY    25,26
  91. ApaBI      8 GCA_nnnnn'TGC  -5          !                                    >    27
  92. ApaLI      1 G'TGCA_C       4           !  Alw44I,SnoI,VneI                  >EJKNUX    28
  93. ;ApyI      2 CC'w_GG        1           !  BsiLI,BstNI,BstOI,Ecl136I,EcoRII,MvaI  >M    29
  94. AscI       2 GG'CGCG_CC     4           !                                    >N    30
  95. AseI       2 AT'TA_AT       2           !  AsnI,VspI                         >NU    31
  96. ;AsnI      2 AT'TA_AT       2           !  AseI,VspI                         >M    32
  97. ;AspI      4 GACn'n_nGTC    1           !  Tth111I                           >M    33
  98. ;Asp700I   5 GAAnn'nnTTC    0           !  XmnI                              >M    34
  99. ;Asp718I   1 G'GTAC_C       4           !  Acc65I,KpnI                       >M    35
  100. ;AspHI     5 G_wGCw'C      -4           !  Alw21I,HgiAI                      >M    36
  101. ;AsuI      1 G'GnC_C        3           !  BsiZI,Cfr13I,NspIV,Sau96I         >R    37
  102. ;AsuII     2 TT'CG_AA       2           !  Bpu14I,BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI  >J    5,38
  103. AvaI       1 C'yCGr_G       4           !  Ama87I,BcoI,Eco88I,NspIII         >ABEIJKLMNOPRSUVX    39,40
  104. AvaII      1 G'GwC_C        3           !  Bme18I,Eco47I,NspHII,SinI         >ABDEIJKMNPRSXY    39,40,41,42
  105. ;AvaIII    3 ATGCAT         ?           !  EcoT22I,Mph1103I,NsiI,Ppu10I,Zsp2I  >J    43,44
  106. ;AviII     3 TGC'GCA        0           !  AosI,FdiII,FspI,MstI              >M    23
  107. AvrII      1 C'CTAG_G       4           !  BlnI                              >N    45,46
  108. ;AxyI      2 CC'TnA_GG      3           !  AocI,Bse21I,Bsu36I,CvnI,Eco81I,MstII,SauI  >JV    47
  109. BaeI       5 ACnnnnGTAyC    ?           !                                    >    48
  110. ;BalI      3 TGG'CCA        0           !  MscI                              >AIJKRSVX    26,49
  111. BamHI      1 G'GATC_C       4           !  BstI                              >ABDEFHIJKLMNOPQRSUVWXY    50,51,52,53
  112. BanI       1 G'GyrC_C       4           !  AccB1I,Eco64I                     >DEIJMNOPRUVXY    1,54
  113. BanII      5 G_rGCy'C      -4           !  Eco24I                            >BEIJKLMNOPRSUVX    1
  114. ;BanIII    2 AT'CG_AT       2           !  BscI,BsiXI,Bsp106I,BspDI,Bsu15I,ClaI  >OU    1,55
  115. ;BbeI      5 G_GCGC'C      -4           !  EheI,KasI,NarI,NunII              >K    56
  116. ;BbiII     2 Gr'CG_yC       2           !  AcyI,BsaHI,Hin1I                  >AK    57
  117. ;BbrPI     3 CAC'GTG        0           !  Eco72I,PmaCI,PmlI                 >M    58
  118. BbsI       8 GAAGACnn'nnnn_   4         !                                    >N    59
  119. ;BbuI      5 G_CATG'C      -4           !  PaeI,SphI                         >RV    60
  120. BbvI      13 GCAGCnnnnnnnn'nnnn_   4    !  Bst71I                            >EIJNX    50,61,62,63
  121. ;BbvII     8 GAAGACnn'nnnn_   4         !  BbsI                              >    64
  122. BccI       2 CCATC          ?           !                                    >    65
  123. BcefI     17 ACGGCnnnnnnnnnnnn'n_   1   !                                    >    66
  124. BcgI      24 CGAnnnnnnTGCnnnnnnnnnn_nn'  -2    !                                    >N    67
  125. BcgI      24 GCAnnnnnnTCGnnnnnnnnnn_nn'  -2    !                                    >N    67
  126. BclI       1 T'GATC_A       4           !  BsiQI,FbaI                        >BDFIJLMNOPRSUVXY    68
  127. ;BcnI      2 CC's_GG        1           !  NciI                              >DFKY    69,70,71,72,73,74
  128. ;BcoI      1 C'yCGr_G       4           !  Ama87I,AvaI,Eco88I,NspIII         >W    75
  129. BetI       1 w'CCGG_w       4           !                                    >    48
  130. ;BfrI      1 C'TTAA_G       4           !  AflII,Esp4I                       >M    76
  131. BglI       7 GCCn_nnn'nGGC  -3          !                                    >ABDEFHIJLMNOPQRSUVWXY    77,78,79,80,81
  132. BglII      1 A'GATC_T       4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    77,81,82
  133. ;BinI      9 GGATCnnnn'n_   1           !  AlwI                              >    83
  134. ;BlnI      1 C'CTAG_G       4           !  AvrII                             >K    84
  135. ;Bme18I    1 G'GwC_C        3           !  AvaII,Eco47I,NspHII,SinI          >DY    85
  136. ;BmyI      5 G_dGCh'C      -4           !  Bsp1286I,NspII,SduI               >M    86
  137. ;BpmI     22 CTGGAGnnnnnnnnnnnnnn_nn'  -2    !  GsuI                              >N    87
  138. Bpu10I     2 CC'TnAGC       7           !                                    >    88
  139. ;Bpu14I    2 TT'CG_AA       2           !  AsuII,BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI  >DY    7
  140. Bpu1102I   2 GC'TnA_GC      3           !  CelII,EspI                        >DFNY    89
  141. BsaI       7 GGTCTCn'nnnn_   4          !  Eco31I                            >N    30,90
  142. BsaAI      3 yAC'GTr        0           !                                    >N    91
  143. BsaBI      5 GATnn'nnATC    0           !  BsiBI,MamI                        >N    92
  144. BsaHI      2 Gr'CG_yC       2           !  AcyI,BbiII,Hin1I                  >N    30,93
  145. BsaJI      1 C'CnnG_G       4           !                                    >N    90
  146. ;BscI      2 AT'CG_AT       2           !  BanIII,BsiXI,Bsp106I,BspDI,Bsu15I,ClaI  >JL    94,95
  147. ;BscBI     3 GGn'nCC        0           !  NlaIV                             >I    96
  148. ;BscCI     3 GAATGC         ?           !  BsmI                              >W    96
  149. ;Bse21I    2 CC'TnA_GG      3           !  AocI,AxyI,Bsu36I,CvnI,Eco81I,MstII,SauI  >DY    97,98
  150. ;BseAI     1 T'CCGG_A       4           !  AccIII,BsiMI,Bsp13I,BspEI,Kpn2I,MroI  >M    99
  151. ;BsePI     3 GCGCGC         ?           !  BssHII                            >    100
  152. BsgI      22 GTGCAGnnnnnnnnnnnnnn_nn'  -2    !                                    >N    101
  153. ;BshI      2 GGCC           ?           !  BsuRI,HaeIII,PalI                 >W    96
  154. BsiI       1 C'TCGTG        6           !                                    >    102
  155. ;BsiBI     5 GATnn'nnATC    0           !  BsaBI,MamI                        >W    103
  156. ;BsiCI     2 TT'CG_AA       2           !  AsuII,Bpu14I,Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI  >UW    96
  157. BsiEI      4 CG_ry'CG      -2           !  McrI                              >NW    104
  158. ;BsiLI     2 CC'w_GG        1           !  ApyI,BstNI,BstOI,Ecl136I,EcoRII,MvaI  >W    96
  159. ;BsiMI     1 T'CCGG_A       4           !  AccIII,BseAI,Bsp13I,BspEI,Kpn2I,MroI  >W    96
  160. ;BsiQI     1 T'GATC_A       4           !  BclI,FbaI                         >W    96
  161. BsiWI      1 C'GTAC_G       4           !  Pfl23II,SplI                      >MNUW    105
  162. ;BsiXI     2 AT'CG_AT       2           !  BanIII,BscI,Bsp106I,BspDI,Bsu15I,ClaI  >W    96
  163. ;BsiYI     7 CCnn_nnn'nnGG  -3          !  BslI                              >MUW    106
  164. ;BsiZI     1 G'GnC_C        3           !  AsuI,Cfr13I,NspIV,Sau96I          >W    96
  165. BslI       7 CCnn_nnn'nnGG  -3          !  BsiYI                             >N    107
  166. BsmI       7 GAATG_Cn'     -2           !  BscCI                             >DEJLMNOUVXY    108
  167. BsmAI      6 GTCTCn'nnnn_   4           !  Alw26I                            >NU    109
  168. ;Bsp13I    1 T'CCGG_A       4           !  AccIII,BseAI,BsiMI,BspEI,Kpn2I,MroI  >DY    7
  169. ;Bsp50I    2 CG'CG          0           !  AccII,BstUI,MvnI,ThaI             >DFY    110,111
  170. ;Bsp68I    3 TCG'CGA        0           !  NruI,SpoI                         >DFY    110,111
  171. ;Bsp106I   2 AT'CG_AT       2           !  BanIII,BscI,BsiXI,BspDI,Bsu15I,ClaI  >E    112
  172. ;Bsp119I   2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,BstBI,Csp45I,LspI,NspV,SfuI  >DFY    111,113
  173. ;Bsp120I   1 G'GGCC_C       4           !  ApaI                              >DFY    111,114
  174. ;Bsp143I   2 GATC           ?           !  BspAI,DpnII,MboI,NdeII,Sau3AI     >DFY    115
  175. ;Bsp143II   5 r_GCGC'y      -4          !  HaeII                             >DFY    115
  176. Bsp1286I   5 G_dGCh'C      -4           !  BmyI,NspII,SduI                   >EKNRUX    5,116,117
  177. ;BspAI     0 'GATC_         4           !  Bsp143I,DpnII,MboI,NdeII,Sau3AI   >J    118
  178. ;BspCI     4 CG_AT'CG      -2           !  PvuI,XorII                        >E    119
  179. ;BspDI     2 AT'CG_AT       2           !  BanIII,BscI,BsiXI,Bsp106I,Bsu15I,ClaI  >N    101
  180. BspEI      1 T'CCGG_A       4           !  AccIII,BseAI,BsiMI,Bsp13I,Kpn2I,MroI  >N    120
  181. BspGI      3 CTGGAC         ?           !                                    >    121
  182. BspHI      1 T'CATG_A       4           !                                    >NU    122
  183. BspMI     10 ACCTGCnnnn'nnnn_   4       !                                    >NU    123
  184. ;BspMII    1 T'CCGG_A       4           !  AccIII,BseAI,BsiMI,Bsp13I,BspEI,Kpn2I,MroI  >    30,123
  185. BspWI      7 GCnn_nnn'nnGC  -3          !                                    >M    121
  186. BsrI       6 ACTG_Gn'      -2           !                                    >N    124
  187. BsrBI      3 GAG'CGG        6           !                                    >    125
  188. BssHII     1 G'CGCG_C       4           !                                    >BDEJLMNOQRUVXY    100,126
  189. ;BssT1I    1 C'CwwG_G       4           !  Eco130I,EcoT14I,StyI              >DY    7
  190. ;BstI      1 G'GATC_C       4           !  BamHI                             >JP    127,128
  191. ;Bst71I   13 GCAGCnnnnnnnn'nnnn_   4    !  BbvI                              >R    129
  192. Bst1107I   3 GTA'TAC        0           !                                    >DFNY    130
  193. ;BstBI     2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,Bsp119I,Csp45I,LspI,NspV,SfuI  >N    30,92
  194. BstEII     1 G'GTnAC_C      5           !  BstPI,Eco91I,EcoO65I              >BEJLMNOPRSUVX    131,132
  195. ;BstNI     2 CC'w_GG        1           !  ApyI,BsiLI,BstOI,Ecl136I,EcoRII,MvaI  >EJNX    133,134
  196. ;BstOI     2 CC'w_GG        1           !  ApyI,BsiLI,BstNI,Ecl136I,EcoRII,MvaI  >R    92
  197. ;BstPI     1 G'GTnAC_C      5           !  BstEII,Eco91I,EcoO65I             >K    135
  198. ;BstUI     2 CG'CG          0           !  AccII,Bsp50I,MvnI,ThaI            >NU    92
  199. BstXI      8 CCAn_nnnn'nTGG  -4         !                                    >BEJKLMNOQRUVX    100,136
  200. BstYI      1 r'GATC_y       4           !  MflI,XhoII                        >NU    137
  201. ;BstZI     1 C'GGCC_G       4           !  EagI,EclXI,Eco52I,XmaIII          >R    92,138
  202. ;Bsu15I    2 AT'CG_AT       2           !  BanIII,BscI,BsiXI,Bsp106I,BspDI,ClaI  >DFY    139,140
  203. Bsu36I     2 CC'TnA_GG      3           !  AocI,AxyI,Bse21I,CvnI,Eco81I,MstII,SauI  >NR    141
  204. ;BsuRI     2 GG'CC          0           !  BshI,HaeIII,PalI                  >DFJY    142,143,144
  205. Cac8I      3 GCn'nGC        0           !                                    >    145
  206. ;CauII     2 CC's_GG        1           !  BcnI,NciI                         >    146,147,148
  207. ;CcrI      1 C'TCGA_G       4           !  PaeR7I,SlaI,XhoI                  >X    149
  208. ;CelII     2 GC'TnA_GC      3           !  Bpu1102I,EspI                     >LM    23,150
  209. ;CfoI      3 G_CG'C        -2           !  HhaI,Hin6I,HinP1I                 >BIJLMRV    151
  210. ;CfrI      1 y'GGCC_r       4           !  EaeI                              >    74,152,153,154
  211. ;Cfr9I     1 C'CCGG_G       4           !  PspAI,SmaI,XmaI                   >DFOUY    154,155,156
  212. Cfr10I     1 r'CCGG_y       4           !                                    >ADFKMNOUY    74,154,156,157
  213. ;Cfr13I    1 G'GnC_C        3           !  AsuI,BsiZI,NspIV,Sau96I           >DFKOUY    156,158
  214. ;Cfr42I    4 CC_GC'GG      -2           !  Kpn378I,KspI,MraI,SacII,Sfr303I,SstII  >DFY    159
  215. ClaI       2 AT'CG_AT       2           !  BanIII,BscI,BsiXI,Bsp106I,BspDI,Bsu15I  >ABDJKMNPQRSVXY    160,161,162
  216. ;CpoI      2 CG'GwC_CG      3           !  CspI,RsrII                        >DFKY    163,164
  217. ;CspI      2 CG'GwC_CG      3           !  CpoI,RsrII                        >ORV    165
  218. ;Csp6I     1 G'TA_C         2           !  AfaI,RsaI                         >DFY    166
  219. ;Csp45I    2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,Bsp119I,BstBI,LspI,NspV,SfuI  >ORV    167
  220. CviJI      2 rG'Cy          0           !                                    >    168,169
  221. CviRI      2 TG'CA          0           !                                    >    170,171
  222. ;CvnI      2 CC'TnA_GG      3           !  AocI,AxyI,Bse21I,Bsu36I,Eco81I,MstII,SauI  >B    172,173
  223. DdeI       1 C'TnA_G        3           !                                    >BEIJLMNOPRUVX    174,175,176
  224. DpnI       2 GA'TC          0           !                                    >ABEIJLMNRSUVX    177,178,179
  225. ;DpnII     0 'GATC_         4           !  Bsp143I,BspAI,MboI,NdeII,Sau3AI   >NU    177,179,180
  226. DraI       3 TTT'AAA        0           !                                    >ABDEFIJKLMNOPQRSUVXY    181
  227. ;DraII     2 rG'GnC_Cy      3           !  EcoO109I,PssI                     >EJM    182,183,184
  228. DraIII     6 CAC_nnn'GTG   -3           !                                    >EMNUX    182,183,184
  229. DrdI       7 GACnn_nn'nnGTC  -2         !                                    >N    185
  230. DrdII      3 GAACCA         ?           !                                    >    186
  231. DsaI       1 C'CryG_G       4           !                                    >M    187
  232. ;DsaV      0 'CCnGG_        5           !  ScrFI                             >M    187
  233. EaeI       1 y'GGCC_r       4           !                                    >EJKLMNUVX    188,189
  234. ;EagI      1 C'GGCC_G       4           !  BstZI,EclXI,Eco52I,XmaIII         >N    190
  235. ;Eam1104I   7 CTCTTCn'nnn_   3          !  EarI,Ksp632I                      >DFY    130
  236. Eam1105I   6 GACnn_n'nnGTC  -1          !                                    >DFNY    191
  237. EarI       7 CTCTTCn'nnn_   3           !  Eam1104I,Ksp632I                  >N    192
  238. EciI       3 TCCGCC         ?           !                                    >    193
  239. ;Ecl136I   2 CCwGG          ?           !  ApyI,BsiLI,BstNI,BstOI,EcoRII,MvaI  >DY    194
  240. ;Ecl136II   3 GAG'CTC        0          !  SacI,SstI                         >FN    195
  241. ;EclXI     1 C'GGCC_G       4           !  BstZI,EagI,Eco52I,XmaIII          >M    196
  242. ;Eco24I    5 G_rGCy'C      -4           !  BanII                             >DFY    197,198
  243. ;Eco31I    7 GGTCTCn'nnnn_   4          !  BsaI                              >DFY    199
  244. ;Eco32I    3 GAT'ATC        0           !  EcoRV                             >DFY    154,200
  245. ;Eco47I    1 G'GwC_C        3           !  AvaII,Bme18I,NspHII,SinI          >DFOUY    201,202
  246. Eco47III   3 AGC'GCT        0           !                                    >DFKLMNORUY    201
  247. ;Eco52I    1 C'GGCC_G       4           !  BstZI,EagI,EclXI,XmaIII           >DEFKORUY    154,202
  248. Eco57I    22 CTGAAGnnnnnnnnnnnnnn_nn'  -2    !                                    >DFNY    203
  249. ;Eco64I    1 G'GyrC_C       4           !  AccB1I,BanI                       >DFY    204,205
  250. ;Eco72I    3 CAC'GTG        0           !  BbrPI,PmaCI,PmlI                  >DFY    206
  251. ;Eco81I    2 CC'TnA_GG      3           !  AocI,AxyI,Bse21I,Bsu36I,CvnI,MstII,SauI  >ADFKOUY    207
  252. ;Eco88I    1 C'yCGr_G       4           !  Ama87I,AvaI,BcoI,NspIII           >DFY    111,208
  253. ;Eco91I    1 G'GTnAC_C      5           !  BstEII,BstPI,EcoO65I              >DFY    209,210
  254. ;Eco105I   3 TAC'GTA        0           !  SnaBI                             >DFOUY    195
  255. ;Eco130I   1 C'CwwG_G       4           !  BssT1I,EcoT14I,StyI               >DFUY    114,211
  256. ;Eco147I   3 AGG'CCT        0           !  AatI,StuI                         >DFY    114,212
  257. EcoNI      5 CCTnn'n_nnAGG   1          !                                    >NU    213
  258. ;EcoO65I   1 G'GTnAC_C      5           !  BstEII,BstPI,Eco91I               >K    214,215
  259. EcoO109I   2 rG'GnC_Cy      3           !  DraII,PssI                        >DFJKLNOUVXY    216
  260. EcoRI      1 G'AATT_C       4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    217,218,219
  261. EcoRII     0 'CCwGG_        5           !  ApyI,BsiLI,BstNI,BstOI,Ecl136I,MvaI  >BEJOUV    220,221,222
  262. EcoRV      3 GAT'ATC        0           !  Eco32I                            >ABDEIJKLMNOPQRSUVWXY    223,224,225
  263. ;EcoT14I   1 C'CwwG_G       4           !  BssT1I,Eco130I,StyI               >AK    226
  264. ;EcoT22I   5 A_TGCA'T      -4           !  AvaIII,Mph1103I,NsiI,Ppu10I,Zsp2I  >KOU    227
  265. ;EheI      3 GGC'GCC        0           !  BbeI,KasI,NarI,NunII              >FOU    228,229
  266. ;EspI      2 GC'TnA_GC      3           !  Bpu1102I,CelII                    >JU    230
  267. Esp3I      7 CGTCTCn'nnnn_   4          !                                    >DFNY    110,111
  268. ;Esp4I     1 C'TTAA_G       4           !  AflII,BfrI                        >F    195
  269. FauI       9 CCCGCnnnn'nn_   2          !                                    >    8,231
  270. ;FbaI      3 TGATCA         ?           !  BclI,BsiQI                        >K    232
  271. ;FdiII     3 TGC'GCA        0           !  AosI,AviII,FspI,MstI              >U    233,234
  272. FinI       2 GTCCC          ?           !                                    >    30
  273. Fnu4HI     2 GC'n_GC        1           !                                    >N    235
  274. ;FnuDII    2 CG'CG          0           !  AccII,Bsp50I,BstUI,MvnI,ThaI      >    236
  275. FokI      14 GGATGnnnnnnnnn'nnnn_   4   !                                    >DEFIKMNRUVXY    237,238,239,240
  276. FseI       6 GG_CCGG'CC    -4           !                                    >    241
  277. FsiI       1 r'AATT_y       4           !                                    >    30
  278. FspI       3 TGC'GCA        0           !  AosI,AviII,FdiII,MstI             >NSU    108,242
  279. GdiII      1 y'GGCCG        6           !                                    >    243
  280. GsuI      22 CTGGAGnnnnnnnnnnnnnn_nn'  -2    !  BpmI                              >DFNY    203,244
  281. HaeI       3 wGG'CCw        0           !                                    >    245
  282. HaeII      5 r_GCGC'y      -4           !  Bsp143II                          >BDEIJKLMNOPRSUVXY    246,247
  283. HaeIII     2 GG'CC          0           !  BshI,BsuRI,PalI                   >ABDHIJKLMNOPQRSUVXY    143,248,249
  284. ;HapII     1 C'CG_G         2           !  HpaII,MspI                        >IK    250,251
  285. HgaI      10 GACGCnnnnn'nnnnn_   5      !                                    >DNUXY    250,252,253
  286. HgiAI      5 G_wGCw'C      -4           !  Alw21I,AspHI                      >NX    254
  287. ;HgiCI     1 G'GyrC_C       4           !  AccB1I,BanI,Eco64I                >    54,255
  288. HgiEII     6 ACCnnnnnnGGT   ?           !                                    >    255
  289. ;HgiJII    5 G_rGCy'C      -4           !  BanII,Eco24I                      >    256
  290. HhaI       3 G_CG'C        -2           !  CfoI,Hin6I,HinP1I                 >BEJKNOPRSUX    257,258
  291. ;Hin1I     2 Gr'CG_yC       2           !  AcyI,BbiII,BsaHI                  >DFOUY    259
  292. ;Hin6I     1 G'CG_C         2           !  CfoI,HhaI,HinP1I                  >DFY    259
  293. ;HinP1I    1 G'CG_C         2           !  CfoI,HhaI,Hin6I                   >NX    260
  294. HincII     3 GTy'rAC        0           !  HindII                            >ABDEFHIJKLNOPQRSUVXY    261
  295. ;HindII    3 GTy'rAC        0           !  HincII                            >EM    262,263,264,265
  296. HindIII    1 A'AGCT_T       4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    262,263,266
  297. HinfI      1 G'AnT_C        3           !                                    >ABDEFIJKLMNOPQRSUVWXY    267,268,269
  298. HpaI       3 GTT'AAC        0           !                                    >ABDEFIJKLMNOPQRSUVXY    168,270,271
  299. ;HpaII     1 C'CG_G         2           !  HapII,MspI                        >ABDEFJLMNOPQRSUVXY    248,270,271
  300. HphI      13 GGTGAnnnnnnn_n'  -1        !                                    >NUVX    268,272,273
  301. ;KasI      1 G'GCGC_C       4           !  BbeI,EheI,NarI,NunII              >N    274
  302. KpnI       5 G_GTAC'C      -4           !  Acc65I,Asp718I                    >ABDEFIJKLMNOPQRSUVXY    275,276,277
  303. ;Kpn2I     1 T'CCGG_A       4           !  AccIII,BseAI,BsiMI,Bsp13I,BspEI,MroI  >F    114,278
  304. ;Kpn378I   4 CC_GC'GG      -2           !  Cfr42I,KspI,MraI,SacII,Sfr303I,SstII  >DY    279
  305. ;KspI      4 CC_GC'GG      -2           !  Cfr42I,Kpn378I,MraI,SacII,Sfr303I,SstII  >EM    280
  306. ;Ksp632I   7 CTCTTCn'nnn_   3           !  Eam1104I,EarI                     >M    281
  307. ;LspI      2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,Bsp119I,BstBI,Csp45I,NspV,SfuI  >JL    94,282
  308. ;MaeI      1 C'TA_G         2           !  RmaI                              >M    283
  309. MaeII      1 A'CG_T         2           !                                    >M    283
  310. MaeIII     0 'GTnAC_        5           !                                    >M    283
  311. ;MamI      5 GATnn'nnATC    0           !  BsaBI,BsiBI                       >M    284
  312. ;MboI      0 'GATC_         4           !  Bsp143I,BspAI,DpnII,NdeII,Sau3AI  >BDEFIJKNPQRSVXY    285
  313. MboII     13 GAAGAnnnnnnn_n'  -1        !                                    >BDFIJKNOPQRSUVXY    285,286,287,288
  314. ;McrI      4 CG_ry'CG      -2           !  BsiEI                             >M    289
  315. ;MfeI      1 C'AATT_G       4           !  MunI                              >    290,291
  316. ;MflI      1 r'GATC_y       4           !  BstYI,XhoII                       >AK    292
  317. MluI       1 A'CGCG_T       4           !                                    >ABDEFIJKLMNOPQRSUVXY    237
  318. MlyI      10 GACTCnnnnn'    0           !                                    >    282
  319. MmeI      26 TCCrACnnnnnnnnnnnnnnnnnn_nn'  -2    !                                    >    293
  320. MnlI      11 CCTCnnnnnn_n'  -1          !                                    >EJNUX    63,294,295
  321. ;Mph1103I   5 A_TGCA'T      -4          !  AvaIII,EcoT22I,NsiI,Ppu10I,Zsp2I  >F    296
  322. ;MraI      3 CCGCGG         ?           !  Cfr42I,Kpn378I,KspI,SacII,Sfr303I,SstII  >J    297
  323. ;MroI      1 T'CCGG_A       4           !  AccIII,BseAI,BsiMI,Bsp13I,BspEI,Kpn2I  >MOU    298
  324. MscI       3 TGG'CCA        0           !  BalI                              >BNU    163,299
  325. MseI       1 T'TA_A         2           !  Tru9I                             >NU    300
  326. MslI       5 CAynnnnrTG     ?           !                                    >    301
  327. MspI       1 C'CG_G         2           !  HapII,HpaII                       >ABDEFIJKLMNOPQRSUVWXY    126,302,303
  328. ;MstI      3 TGC'GCA        0           !  AosI,AviII,FdiII,FspI             >X    61,304
  329. ;MstII     2 CC'TnA_GG      3           !  AocI,AxyI,Bse21I,Bsu36I,CvnI,Eco81I,SauI  >EX    63
  330. MunI       1 C'AATT_G       4           !                                    >FN    305
  331. ;MvaI      2 CC'w_GG        1           !  ApyI,BsiLI,BstNI,BstOI,Ecl136I,EcoRII  >ADFKMOUY    306,307
  332. ;MvnI      2 CG'CG          0           !  AccII,Bsp50I,BstUI,ThaI           >DMY    308
  333. ;MwoI      7 GCnn_nnn'nnGC  -3          !  BspWI                             >    309
  334. NaeI       3 GCC'GGC        0           !                                    >EKLMNOUVX    310
  335. NarI       2 GG'CG_CC       2           !  BbeI,EheI,KasI,NunII              >BDEJMNOPRUVXY    311
  336. NciI       2 CC's_GG        1           !  BcnI                              >BEJLMNOUVWX    312,313
  337. NcoI       1 C'CATG_G       4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    100
  338. NdeI       2 CA'TA_TG       2           !                                    >BEFKLMNPSUVX    314,315,316
  339. ;NdeII     0 'GATC_         4           !  Bsp143I,BspAI,DpnII,MboI,Sau3AI   >BM    317
  340. NheI       1 G'CTAG_C       4           !                                    >BEJKLMNOPRUVX    318
  341. NlaIII     4 _CATG'        -4           !                                    >NU    319
  342. NlaIV      3 GGn'nCC        0           !  BscBI                             >NU    319
  343. NotI       2 GC'GGCC_GC     4           !                                    >ABDEFIJKLMNOPQRSUVWXY    320,321
  344. NruI       3 TCG'CGA        0           !  Bsp68I,SpoI                       >BDEIJKLMNOPQUVWXY    322
  345. NsiI       5 A_TGCA'T      -4           !  AvaIII,EcoT22I,Mph1103I,Ppu10I,Zsp2I  >BELMNRVX    323
  346. NspI       5 r_CATG'y      -4           !  NspHI                             >AKMU    324
  347. ;NspII     5 G_dGCh'C      -4           !  BmyI,Bsp1286I,SduI                >J    324
  348. ;NspIII    1 C'yCGr_G       4           !  Ama87I,AvaI,BcoI,Eco88I           >JP    324
  349. ;NspIV     1 G'GnC_C        3           !  AsuI,BsiZI,Cfr13I,Sau96I          >J    324
  350. NspV       2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,Bsp119I,BstBI,Csp45I,LspI,SfuI  >ABKOP    324,325
  351. NspBII     3 CmG'CkG        0           !                                    >JU    326
  352. ;NspHI     5 r_CATG'y      -4           !  NspI                              >J    326
  353. ;NspHII    2 GGwCC          ?           !  AvaII,Bme18I,Eco47I,SinI          >J    326
  354. ;NunII     2 GG'CG_CC       2           !  BbeI,EheI,KasI,NarI               >J    100
  355. PacI       5 TTA_AT'TAA    -2           !                                    >N    327
  356. ;PaeI      5 G_CATG'C      -4           !  BbuI,SphI                         >DFY    328
  357. ;PaeR7I    1 C'TCGA_G       4           !  CcrI,SlaI,XhoI                    >NX    329,330
  358. ;PalI      2 GG'CC          0           !  BshI,BsuRI,HaeIII                 >EJPV    331,332
  359. ;Pfl23II   1 C'GTAC_G       4           !  BsiWI,SplI                        >F    111,333
  360. Pfl1108I   3 TCGTAG         ?           !                                    >    334
  361. PflMI      7 CCAn_nnn'nTGG  -3          !  AccB7I,Van91I                     >NU    30
  362. PleI       9 GAGTCnnnn'n_   1           !                                    >NU    335
  363. ;PmaCI     3 CAC'GTG        0           !  BbrPI,Eco72I,PmlI                 >AK    336
  364. PmeI       4 GTTT'AAAC      0           !                                    >N    48
  365. PmlI       3 CAC'GTG        0           !  BbrPI,Eco72I,PmaCI                >NU    337
  366. ;Ppu10I    1 A'TGCA_T       4           !  AvaIII,EcoT22I,Mph1103I,NsiI,Zsp2I  >F    111,115
  367. PpuMI      2 rG'GwC_Cy      3           !  Psp5II                            >NU    338
  368. PshAI      5 GACnn'nnGTC    0           !                                    >K    339
  369. ;Psp5I     3 CAGCTG         ?           !  PvuII                             >DY    296
  370. ;Psp5II    3 rGGwCCy        ?           !  PpuMI                             >F    296
  371. ;PspAI     1 C'CCGG_G       4           !  Cfr9I,SmaI,XmaI                   >E    340
  372. ;PssI      5 rG_GnC'Cy     -3           !  DraII,EcoO109I                    >I    151,341
  373. PstI       5 C_TGCA'G      -4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    276,342,343
  374. PvuI       4 CG_AT'CG      -2           !  BspCI,XorII                       >ABDEFJKLMNOPQRSUVXY    344
  375. PvuII      3 CAG'CTG        0           !  Psp5I                             >ABDEFIJKLMNOPQRSUVWXY    344,345
  376. RleAI     18 CCCACAnnnnnnnnn_nnn'  -3   !                                    >    346
  377. RmaI       1 C'TA_G         2           !  MaeI                              >N    347
  378. RsaI       2 GT'AC          0           !  AfaI,Csp6I                        >ABDEIJLMNOPQRSUVWXY    348
  379. RsrII      2 CG'GwC_CG      3           !  CpoI,CspI                         >BEJMNUX    349
  380. ;RtrI      1 G'TCGA_C       4           !  SalI                              >DY    350
  381. ;SacI      5 G_AGCT'C      -4           !  Ecl136II,SstI                     >ADEIJKLMNOPQRSUVWXY    351
  382. SacII      4 CC_GC'GG      -2           !  Cfr42I,Kpn378I,KspI,MraI,Sfr303I,SstII  >EIJLNOPRUVX    351
  383. SalI       1 G'TCGA_C       4           !  RtrI                              >ABDEFHIJKLMNOPQRSUVXY    352
  384. SapI       8 GCTCTTCn'nnn_   3          !                                    >    30
  385. ;SauI      2 CC'TnA_GG      3           !  AocI,AxyI,Bse21I,Bsu36I,CvnI,Eco81I,MstII  >M    353
  386. Sau96I     1 G'GnC_C        3           !  AsuI,BsiZI,Cfr13I,NspIV           >BDEJLMNORVXY    354
  387. Sau3AI     0 'GATC_         4           !  Bsp143I,BspAI,DpnII,MboI,NdeII    >ABDEIJKLMNOPQRSUVXY    355,356
  388. ScaI       3 AGT'ACT        0           !                                    >ABDEFIJKLMNOPRSUVXY    357,358
  389. ScrFI      2 CC'n_GG        1           !  DsaV                              >EMNOSUVX    359
  390. ;SduI      5 G_dGCh'C      -4           !  BmyI,Bsp1286I,NspII               >DFJY    360,361
  391. ;SecI      1 C'CnnG_G       4           !  BsaJI                             >    362
  392. SfaNI     10 GCATCnnnnn'nnnn_   4       !                                    >DNUXY    126,363
  393. SfcI       1 C'TryA_G       4           !                                    >N    364
  394. ;SfeI      1 C'TryA_G       4           !  SfcI                              >    365
  395. SfiI       8 GGCCn_nnn'nGGCC  -3        !                                    >ABDEIJLMNOPQRSUVXY    366
  396. ;Sfr303I   4 CC_GC'GG      -2           !  Cfr42I,Kpn378I,KspI,MraI,SacII,SstII  >DY    7
  397. ;SfuI      2 TT'CG_AA       2           !  AsuII,Bpu14I,BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV  >M    367
  398. SgrAI      2 Cr'CCGG_yG     4           !                                    >M    368
  399. ;SinI      1 G'GwC_C        3           !  AvaII,Bme18I,Eco47I,NspHII        >JLRSV    23,369,370
  400. ;SlaI      1 C'TCGA_G       4           !  CcrI,PaeR7I,XhoI                  >DY    371
  401. SmaI       3 CCC'GGG        0           !  Cfr9I,PspAI,XmaI                  >ABDEFIJKLMNOPQRSUVWXY    155,372,373,374
  402. ;SnaI      3 GTATAC         ?           !  Bst1107I                          >    375
  403. SnaBI      3 TAC'GTA        0           !  Eco105I                           >EJKLMNRVX    376
  404. ;SnoI      1 G'TGCA_C       4           !  Alw44I,ApaLI,VneI                 >BJLMV    94,282
  405. SpeI       1 A'CTAG_T       4           !                                    >BEKLMNORSUVWX    377
  406. SphI       5 G_CATG'C      -4           !  BbuI,PaeI                         >ABDEIJKLMNOPQRSUXY    378
  407. ;SplI      1 C'GTAC_G       4           !  BsiWI,Pfl23II                     >AK    379
  408. ;SpoI      3 TCG'CGA        0           !  Bsp68I,NruI                       >R    273
  409. SrfI       4 GCCC'GGGC      0           !                                    >    119
  410. Sse8387I   6 CC_TGCA'GG    -4           !                                    >AK    380
  411. SspI       3 AAT'ATT        0           !                                    >BDEFKLMNORUVXY    381
  412. SstI       5 G_AGCT'C      -4           !  Ecl136II,SacI                     >B    382,383
  413. ;SstII     4 CC_GC'GG      -2           !  Cfr42I,Kpn378I,KspI,MraI,SacII,Sfr303I  >B    382
  414. StuI       3 AGG'CCT        0           !  AatI,Eco147I                      >ABEIJKLMNPRVX    384
  415. StyI       1 C'CwwG_G       4           !  BssT1I,Eco130I,EcoT14I            >BDEJMNRUVXY    385
  416. SwaI       4 ATTT'AAAT      0           !                                    >M    386
  417. TaqI       1 T'CG_A         2           !  TthHB8I                           >ABDEFIJLMNOPQRSUVWXY    387,388
  418. TaqII-1   17 GACCGAnnnnnnnnn_nn'  -2    !                                    >    5,389
  419. TaqII-2   17 CACCCAnnnnnnnnn_nn'  -2    !                                    >    5,389
  420. TfiI       1 G'AwT_C        3           !                                    >N    107
  421. ThaI       2 CG'CG          0           !  AccII,Bsp50I,BstUI,MvnI           >BI    390
  422. ;Tru9I     1 T'TA_A         2           !  MseI                              >DY    391
  423. Tsp45I     2 GTsAC          ?           !                                    >    392
  424. TspEI      2 AATT           ?           !                                    >    393
  425. Tth111I    4 GACn'n_nGTC    1           !  AspI                              >EIJKNPRUVX    394
  426. Tth111II  17 CAArCAnnnnnnnnn_nn'  -2    !                                    >    395
  427. ;TthHB8I   1 T'CG_A         2           !  TaqI                              >K    387,396,397,398
  428. ;Van91I    7 CCAn_nnn'nTGG  -3          !  AccB7I,PflMI                      >FM    296
  429. ;VneI      1 G'TGCA_C       4           !  Alw44I,ApaLI,SnoI                 >DY    399
  430. ;VspI      2 AT'TA_AT       2           !  AseI,AsnI                         >DFKRY    400
  431. XbaI       1 T'CTAG_A       4           !                                    >ABDEFHIJKLMNOPQRSUVWXY    401,402
  432. XcmI       8 CCAnnnn_n'nnnnTGG  -1      !                                    >NU    403
  433. XhoI       1 C'TCGA_G       4           !  CcrI,PaeR7I,SlaI                  >ABDEFHIJKLMNOPQRSUVXY    404
  434. ;XhoII     1 r'GATC_y       4           !  BstYI,MflI                        >EMRVX    62,405,406
  435. ;XmaI      1 C'CCGG_G       4           !  Cfr9I,PspAI,SmaI                  >DEINRUVXY    372
  436. XmaIII     1 C'GGCC_G       4           !  BstZI,EagI,EclXI,Eco52I           >BE    26,407
  437. XmnI       5 GAAnn'nnTTC    0           !  Asp700I                           >DEJNUXY    408,409
  438. ;XorII     4 CG_AT'CG      -2           !  BspCI,PvuI                        >B    344,410
  439. ;Zsp2I     5 A_TGCA'T      -4           !  AvaIII,EcoT22I,Mph1103I,NsiI,Ppu10I  >DY    7
  440.  
  441.